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area fraction fractionator tool  (MBF Bioscience)

 
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    MBF Bioscience area fraction fractionator tool
    AAV2.retro-mediated retrograde transport is significantly higher compared to the parent serotype, AAV2. ( a ) Low and ( b ) high power photomicrographs of eGFP-stained, rhesus macaque coronal brain sections following injection of AAV2.retro-eGFP (top panel) or AAV2-eGFP (bottom panel) into the caudate and putamen. Rectangles in ( a ) illustrate the brain regions selected for high power photomicrographs displayed in (b ). ( c ) Cell count analysis demonstrating significantly more GFP + cells in the putamen compared to the caudate, irrespective of serotype, as well as significantly more GFP + cells detected in extra-striatal regions in animals injected with AAV2.retro-eGFP compared to AAV2-eGFP. ( d ) Area fraction <t>fractionator</t> analysis showing a significantly higher area fraction of GFP positivity in the putamen in animals injected with AAV2-eGFP compared to AAV2.retro-eGFP. Error bars in both graphs represent standard error of the mean (SEM). Abbreviations: ACC (anterior cingulate cortex), AMY (amygdala), Cd (caudate), DPFC (dorsal prefrontal cortex), DPMC (dorsal premotor cortex), Put (putamen), SSC (somatosensory cortex), THAL (thalamus). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar 2a = 1 centimeter, Scale bar 2b = 100 microns. Graphs were made in GraphPad Prism for Mac, Version 8.
    Area Fraction Fractionator Tool, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/area+fraction+fractionator+tool/pmc07181773-107-23-27?v=MBF+Bioscience
    Average 90 stars, based on 1 article reviews
    area fraction fractionator tool - by Bioz Stars, 2026-06
    90/100 stars

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    1) Product Images from "Intra-striatal AAV2.retro administration leads to extensive retrograde transport in the rhesus macaque brain: implications for disease modeling and therapeutic development"

    Article Title: Intra-striatal AAV2.retro administration leads to extensive retrograde transport in the rhesus macaque brain: implications for disease modeling and therapeutic development

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-63559-7

    AAV2.retro-mediated retrograde transport is significantly higher compared to the parent serotype, AAV2. ( a ) Low and ( b ) high power photomicrographs of eGFP-stained, rhesus macaque coronal brain sections following injection of AAV2.retro-eGFP (top panel) or AAV2-eGFP (bottom panel) into the caudate and putamen. Rectangles in ( a ) illustrate the brain regions selected for high power photomicrographs displayed in (b ). ( c ) Cell count analysis demonstrating significantly more GFP + cells in the putamen compared to the caudate, irrespective of serotype, as well as significantly more GFP + cells detected in extra-striatal regions in animals injected with AAV2.retro-eGFP compared to AAV2-eGFP. ( d ) Area fraction fractionator analysis showing a significantly higher area fraction of GFP positivity in the putamen in animals injected with AAV2-eGFP compared to AAV2.retro-eGFP. Error bars in both graphs represent standard error of the mean (SEM). Abbreviations: ACC (anterior cingulate cortex), AMY (amygdala), Cd (caudate), DPFC (dorsal prefrontal cortex), DPMC (dorsal premotor cortex), Put (putamen), SSC (somatosensory cortex), THAL (thalamus). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar 2a = 1 centimeter, Scale bar 2b = 100 microns. Graphs were made in GraphPad Prism for Mac, Version 8.
    Figure Legend Snippet: AAV2.retro-mediated retrograde transport is significantly higher compared to the parent serotype, AAV2. ( a ) Low and ( b ) high power photomicrographs of eGFP-stained, rhesus macaque coronal brain sections following injection of AAV2.retro-eGFP (top panel) or AAV2-eGFP (bottom panel) into the caudate and putamen. Rectangles in ( a ) illustrate the brain regions selected for high power photomicrographs displayed in (b ). ( c ) Cell count analysis demonstrating significantly more GFP + cells in the putamen compared to the caudate, irrespective of serotype, as well as significantly more GFP + cells detected in extra-striatal regions in animals injected with AAV2.retro-eGFP compared to AAV2-eGFP. ( d ) Area fraction fractionator analysis showing a significantly higher area fraction of GFP positivity in the putamen in animals injected with AAV2-eGFP compared to AAV2.retro-eGFP. Error bars in both graphs represent standard error of the mean (SEM). Abbreviations: ACC (anterior cingulate cortex), AMY (amygdala), Cd (caudate), DPFC (dorsal prefrontal cortex), DPMC (dorsal premotor cortex), Put (putamen), SSC (somatosensory cortex), THAL (thalamus). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar 2a = 1 centimeter, Scale bar 2b = 100 microns. Graphs were made in GraphPad Prism for Mac, Version 8.

    Techniques Used: Staining, Injection, Cell Counting



    Similar Products

    area fraction fractionator tool  (MBF Bioscience)
    90
    MBF Bioscience area fraction fractionator tool
    AAV2.retro-mediated retrograde transport is significantly higher compared to the parent serotype, AAV2. ( a ) Low and ( b ) high power photomicrographs of eGFP-stained, rhesus macaque coronal brain sections following injection of AAV2.retro-eGFP (top panel) or AAV2-eGFP (bottom panel) into the caudate and putamen. Rectangles in ( a ) illustrate the brain regions selected for high power photomicrographs displayed in (b ). ( c ) Cell count analysis demonstrating significantly more GFP + cells in the putamen compared to the caudate, irrespective of serotype, as well as significantly more GFP + cells detected in extra-striatal regions in animals injected with AAV2.retro-eGFP compared to AAV2-eGFP. ( d ) Area fraction <t>fractionator</t> analysis showing a significantly higher area fraction of GFP positivity in the putamen in animals injected with AAV2-eGFP compared to AAV2.retro-eGFP. Error bars in both graphs represent standard error of the mean (SEM). Abbreviations: ACC (anterior cingulate cortex), AMY (amygdala), Cd (caudate), DPFC (dorsal prefrontal cortex), DPMC (dorsal premotor cortex), Put (putamen), SSC (somatosensory cortex), THAL (thalamus). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar 2a = 1 centimeter, Scale bar 2b = 100 microns. Graphs were made in GraphPad Prism for Mac, Version 8.
    Area Fraction Fractionator Tool, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/area+fraction+fractionator+tool/pmc07181773-107-23-27?v=MBF+Bioscience
    Average 90 stars, based on 1 article reviews
    area fraction fractionator tool - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

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    AAV2.retro-mediated retrograde transport is significantly higher compared to the parent serotype, AAV2. ( a ) Low and ( b ) high power photomicrographs of eGFP-stained, rhesus macaque coronal brain sections following injection of AAV2.retro-eGFP (top panel) or AAV2-eGFP (bottom panel) into the caudate and putamen. Rectangles in ( a ) illustrate the brain regions selected for high power photomicrographs displayed in (b ). ( c ) Cell count analysis demonstrating significantly more GFP + cells in the putamen compared to the caudate, irrespective of serotype, as well as significantly more GFP + cells detected in extra-striatal regions in animals injected with AAV2.retro-eGFP compared to AAV2-eGFP. ( d ) Area fraction fractionator analysis showing a significantly higher area fraction of GFP positivity in the putamen in animals injected with AAV2-eGFP compared to AAV2.retro-eGFP. Error bars in both graphs represent standard error of the mean (SEM). Abbreviations: ACC (anterior cingulate cortex), AMY (amygdala), Cd (caudate), DPFC (dorsal prefrontal cortex), DPMC (dorsal premotor cortex), Put (putamen), SSC (somatosensory cortex), THAL (thalamus). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar 2a = 1 centimeter, Scale bar 2b = 100 microns. Graphs were made in GraphPad Prism for Mac, Version 8.

    Journal: Scientific Reports

    Article Title: Intra-striatal AAV2.retro administration leads to extensive retrograde transport in the rhesus macaque brain: implications for disease modeling and therapeutic development

    doi: 10.1038/s41598-020-63559-7

    Figure Lengend Snippet: AAV2.retro-mediated retrograde transport is significantly higher compared to the parent serotype, AAV2. ( a ) Low and ( b ) high power photomicrographs of eGFP-stained, rhesus macaque coronal brain sections following injection of AAV2.retro-eGFP (top panel) or AAV2-eGFP (bottom panel) into the caudate and putamen. Rectangles in ( a ) illustrate the brain regions selected for high power photomicrographs displayed in (b ). ( c ) Cell count analysis demonstrating significantly more GFP + cells in the putamen compared to the caudate, irrespective of serotype, as well as significantly more GFP + cells detected in extra-striatal regions in animals injected with AAV2.retro-eGFP compared to AAV2-eGFP. ( d ) Area fraction fractionator analysis showing a significantly higher area fraction of GFP positivity in the putamen in animals injected with AAV2-eGFP compared to AAV2.retro-eGFP. Error bars in both graphs represent standard error of the mean (SEM). Abbreviations: ACC (anterior cingulate cortex), AMY (amygdala), Cd (caudate), DPFC (dorsal prefrontal cortex), DPMC (dorsal premotor cortex), Put (putamen), SSC (somatosensory cortex), THAL (thalamus). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar 2a = 1 centimeter, Scale bar 2b = 100 microns. Graphs were made in GraphPad Prism for Mac, Version 8.

    Article Snippet: To assess these serotype-based differences, we quantified the spread of each vector in the injected regions of the caudate and putamen using the Area Fraction Fractionator tool (MBF Bioscience), calculating the area of eGFP + cells per area of each structure.

    Techniques: Staining, Injection, Cell Counting